Lab Exercises: Salivary Glands

salivary1.jpg (167427 bytes) Look at slide 27, the Parotid gland.  This is a large salivary gland that consists of cells that are largely serous.  It is divided into lobes by connective tissue septa.  Clusters of gland cells are organized in Secretory acini.  The cells are filled with granules. The saliva is collected in small ducts that leave the lobules and move out into the connective tissue between the lobes.  Identify gland and duct cells in these photographs.

salivary2.jpg (135835 bytes) This view shows the granulated cytoplasm and the smallest ducts leaving a cluster of glandular cells.

 

 

 

Look at slide 28, the Submandibular Gland. This is a mixed salivary gland.  Most of the glandular cells are serous.  However some are mucus. In the following photograph, identify mucous cells, serous cells and duct cells.  

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salivary4.jpg (133324 bytes)  In some of the acini, the serous cells form a cap (shaped like a half moon) over the centrally located mucous cells.  This is called a SEROUS DEMILUNE. These photographs show the granules in the serous glandular cells as well as the mucous cells in the center of the demilune.

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In the interlobar connective tissue, large EXCRETORY DUCTS can be seen.  These are distinguished by their layers of nuclei and Goblet cells.  The following photographs show views of this type of duct.  Identify the Goblet cells.

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Classify the epithelium in the EXCRETORY DUCTS

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Slide 29 is the sublingual gland, which has more mucous cells than serous cells. This photograph shows a section of the sublingual gland. You can also see serous demilunes in this gland.

 

salivary12.jpg (93231 bytes) This view shows one of the ducts in the sublingual gland called the Striated duct.  The nuclei have been pushed towards the lumen.

The surface specializations found at  the base of these striated duct cells are lateral interdigitations, similar to those found in the kidney proximal tubule cells.  They have sodium/potassium ATPases (pumps) that help with sodium and water conservation.  

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Slide 30 is a section of the submandibular gland stained with Masson's trichrome and Alcian yellow.  The yellow stain detects the mucous cells. They are easy to distinguish.

 

salivary15.jpg (91979 bytes) This view shows a higher magnification of a serous demilune in the submandibular gland.

 

 

 

 

 

salivary16.jpg (133099 bytes) Excretory ducts can also be found in slide 30.   They are distinguished by the the yellow staining Goblet cells.

 

 

 

 

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Higher magnification showing Goblet cells in the EXCRETORY DUCT of the Submandibular gland.

What is the difference between intercalated ducts and striated ducts? 

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Last updated: 06/04/01
© copyright 1998 Gwen V. Childs, Ph.D.
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