|Before cell division, the DNA in our chromosomes replicates so each daughter cell has an identical set of chromosome. In addition, the DNA is responsible for coding for all proteins. Each amino acid is designated by one or more set of triplet nucleotides. The code is produced from one strand of the DNA by a process called "transcription". This produces mRNA which then is sent out of the nucleus where the message is translated into proteins. This can be done in the cytoplasm on clusters of ribosomes, called "polyribosomes". Or it can be done on the membranes of the rough endoplasmic reticulum. The cartoon to the left shows the basic sequence of transcription and translational events.|
The code is actually translated on structures that are also made in the
nucleus, called Ribosomes. These ribosomes provide the structural site where the
mRNA sits. The amino acids for the proteins are carried to the site by
"transfer RNAs,". In the cartoon to the left, these are shown as blue
molecules. Each transfer RNA (tRNA) has a nucleotide triplet which binds to the
complementary sequence on the mRNA (see the three letters at the bottom of each molecule).
The tRNA carries the amino acid at its opposite end. One can trace and detect binding of a particular tRNA-amino acid complex to the mRNA by labeling that amino acid. It will bind to its tRNA. In the case to the left, Phenylalanine is bound to the tRNA which carries the complementary base code AAA (adenine-adenine-adenine). This triplet code would bind to the complementary sequence on mRNA UUU (uracil X3). The mRNA is shown as a green arrow. This cartoon shows the selective binding site on the mRNA which is attached in the ribosome. It also shows the tRNA carrying the Phenylalanine bound at the site In this particular assay which uses a polyuracil mRNA, only phenylalanine-bearing tRNA is bound and detected on the filter.
The cartoon shows the initiation of this process. It begins with the small subunit of the ribosome bound to the mRNA. An initiator tRNA is attracted to the region (carrying a methionine. It binds to the triplet code AUG.
This then attracts the large ribosomal subunit which will bind to the small subunit. Note that it has an A site and a P site. These are different binding sites for the tRNAs. The cartoon below describes the next phase in the process.
In this cartoon, note that the initiator tRNA complex has moved to the P site. This leaves the A site open for the next tRNA. In this case, we have Proline, which carries the complementary code GGC. Note that its binding site on the mRNA is CCG. After binding to the A site, the peptide bond between the methionine and proline forms. The empty tRNA carrying the MET leaves and the tRNA carrying the Proline moves to the P site. The ribosome moves to the next triplet code from 5' to the 3' direction (note arrow on mRNA). The tRNAs are moving from 3' to the 5' direction as the ribosome reads the code
The ribosome continues to read the code from the 5' to the 3' and amino acids are added to the growing peptide chain. This one shows the tRNA carrying the glycine amino acid coded by CCA. Its complementary bases are GGU.
This continues until the stop codon is reached. This is highlighted in red in this figure and the next figure.
The following cartoon shows what happens when the stop codon is reached.
End of translation
Clusters of ribosomes may sit on a mRNA and make proteins, each making a strand of polypeptides. These clusters are called polyribosomes. When they are free in the cytoplasm, they are called free polyribosomes (linked by the mRNA). Or, they may bind to rough endoplasmic reticulum.
Ribosomes are visualized as small (20 X 30 nm) ribonucleoprotein particles. They are formed from two subunits. As you learned in the lecture on the nucleolus , the subunits are produced in the nucleolus in organizing centers on certain chromosomes. The two ribosomal subunits leave the nucleus separately through the nuclear pores . The pores are structured to allow transit of only the subunits. Whole ribosomes are formed outside in the cytoplasm. This prevents protein synthesis from occurring in the nucleus. Why might this be important?
The above photograph shows a group of ribosomes in action. They are connected by a
strand of messenger RNA which runs between the large and small subunits. They read the 3
nucleotide code for an amino acid and the appropriate transfer RNA brings the amino acid
to the growing polypeptide chain. In this photograph, we see the growing peptide chain
radiating at right angles to the mRNA. It extends from the base of the large ribosomal
|The left hand view of this cartoon shows the free
polyribosomes connected by the mRNA. They are arranged in rosettes and these can be seen
in the cytoplasm in conventional electron micrographs. The right hand view shows the
arrangement of polyribosomes on the rough endoplasmic reticulum. Note that the growing
polypeptide chain (which projects down from the large subunit) is inserted through the
membrane and into the cisterna of the rough endoplasmic reticulum.
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This cartoon shows the binding site on the rough endoplasmic reticulum. The membrane of the rough endoplasmic has a receptor that binds the larger subunit of the ribosome. Next to the receptor is a pore that allows newly synthesized proteins to enter and be stored initially in the rough endoplasmic reticulum cisterna or lumen. Note that the ribosomes are still connected to one another outside the rough endoplasmic reticulum by the mRNA which runs between the large and small subunits.
|This electron micrograph shows a high magnification of a longitudinal section through the rough endoplasmic reticulum. The electron dense ribosomes are on its outside surface. Inside the sac (cisterna) is flocculent material, the newly synthesized proteins. The details of ribosomal structure cannot be appreciated in this micrograph. They look like small irregular balls on the outside of the membrane. Note that the sacs of rough endoplasmic reticulum are bridged by a junction. This is shown diagrammatically in the following figure.|
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Learn more about how proteins are handled in the rough endoplasmic reticulum
Study how proteins get to the Golgi Complex
Study the lysosomal sorting and packaging process
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