Cytoskeletal system organizes cell and organelle motility

Describe the Actin Monomer

Describe the Actin Monomer

How is the polymer formed?

Use myosin to detect polarity.

Polymerization to filament

Is ATP hydrolysis required?

EM view of actin filament

Cross linking proteins

Cross linking proteins

Attachment to plasma membrane: Examples

Cortical actin network

How actin binds to membrane in cortical region

What drugs inhibit polymerization?

"A:"

Proteins that cap actin and regulate polymerization.

Function of severing proteins: gelsolin and cofilin

Example of capping proteins

How does the actin cytoskeleton control projections from cells?

Listeria monocytogenes: a model for actin-mediated motility

Actin controls cell process formation

Actin in lammellipodium

Actin controls cell process formation

Focal contacts and stress fibers

Focal contacts

SEM view of cilia and microvilli

How are actin filaments organized inside microvilli?

Core Actin filaments

Slide 29

Terminal Web

Slide 31

Specialized Microvilli

Lateral specializations

  Anchoring junctions at the lateral surface

Adherent junctions

Myosin I and II molecules

Myosin interaction with actin

Requirements for myosin binding and motility

Steps in myosin, actin interactions

How is myosin similar to kinesin?

Where is actin stable and myosin moving?

Myosin moving along actin network.

Where is myosin stable and actin moving?

Sliding filament diagram

What is a sarcomere?

Events involving actin

Myosin and accessory proteins

Other binding proteins

Myosin II also interacts in non-muscle cells at specific sites

Organization in smooth muscle cells.

Key steps involved in locomotion

Locomotion

Myosin II vs Myosin I

Sol to gel transition: how is calcium involved?